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kor agonists u50 488 hydrochloride  (Tocris)


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    Tocris kor agonists u50 488 hydrochloride
    RGS protein family on mechanical pain using an Von Frey Assay and Kappa Opioid Receptor (KOR) unbiased agonist, <t>U50,488.</t> A. Timeline and schematic of the Von Frey procedure. B. Key for male and female mice. C. Mechanical Withdrawal Threshold (MWT) after an injection of over time in male (N=23) and female (N=20) C57BLJ6 mice. D. Average MWT after U50,488 injection over time in RGS6 +/+ and RGS6 -/- male mice (N=11-10). Insert is the average MWT of male RGS6 +/+ and RGS6 -/- at the peak analgesic response point of 180 minutes after injection. E. Average MWT after an injection U50,488 over time in RGS6 +/+ and RGS6 -/- female mice (N= 8-10). Insert is the average MWT of female RGS6 +/+ and RGS6 -/- 180 minutes after injection. F. Average MWT after an injection U50,488 over time in RGS7 +/+ and RGS7 -/- male mice (N=6). Insert is the average MWT of male RGS7 +/+ and RGS7 -/- at 180 minutes after injection. G. Average MWT necessary to elicit a pain response after an injection U50,488 over time in RGS7 +/+ and RGS7 -/- female mice (N=6-7). Insert is the average MWT of female RGS7 +/+ and RGS7 -/- 180 minutes after injection. Data shown as means ± SEM; * p≤0.05, ** p≤0.01, *** p≤0.001, **** p<0.0001.
    Kor Agonists U50 488 Hydrochloride, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u50+488/bio_rxiv__64898__2026__03__04__709600-42-0-11?v=Tocris
    Average 93 stars, based on 30 article reviews
    kor agonists u50 488 hydrochloride - by Bioz Stars, 2026-07
    93/100 stars

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    1) Product Images from "RGS6 regulates Kappa opioid receptor-mediated antinociceptive behaviors"

    Article Title: RGS6 regulates Kappa opioid receptor-mediated antinociceptive behaviors

    Journal: bioRxiv

    doi: 10.64898/2026.03.04.709600

    RGS protein family on mechanical pain using an Von Frey Assay and Kappa Opioid Receptor (KOR) unbiased agonist, U50,488. A. Timeline and schematic of the Von Frey procedure. B. Key for male and female mice. C. Mechanical Withdrawal Threshold (MWT) after an injection of over time in male (N=23) and female (N=20) C57BLJ6 mice. D. Average MWT after U50,488 injection over time in RGS6 +/+ and RGS6 -/- male mice (N=11-10). Insert is the average MWT of male RGS6 +/+ and RGS6 -/- at the peak analgesic response point of 180 minutes after injection. E. Average MWT after an injection U50,488 over time in RGS6 +/+ and RGS6 -/- female mice (N= 8-10). Insert is the average MWT of female RGS6 +/+ and RGS6 -/- 180 minutes after injection. F. Average MWT after an injection U50,488 over time in RGS7 +/+ and RGS7 -/- male mice (N=6). Insert is the average MWT of male RGS7 +/+ and RGS7 -/- at 180 minutes after injection. G. Average MWT necessary to elicit a pain response after an injection U50,488 over time in RGS7 +/+ and RGS7 -/- female mice (N=6-7). Insert is the average MWT of female RGS7 +/+ and RGS7 -/- 180 minutes after injection. Data shown as means ± SEM; * p≤0.05, ** p≤0.01, *** p≤0.001, **** p<0.0001.
    Figure Legend Snippet: RGS protein family on mechanical pain using an Von Frey Assay and Kappa Opioid Receptor (KOR) unbiased agonist, U50,488. A. Timeline and schematic of the Von Frey procedure. B. Key for male and female mice. C. Mechanical Withdrawal Threshold (MWT) after an injection of over time in male (N=23) and female (N=20) C57BLJ6 mice. D. Average MWT after U50,488 injection over time in RGS6 +/+ and RGS6 -/- male mice (N=11-10). Insert is the average MWT of male RGS6 +/+ and RGS6 -/- at the peak analgesic response point of 180 minutes after injection. E. Average MWT after an injection U50,488 over time in RGS6 +/+ and RGS6 -/- female mice (N= 8-10). Insert is the average MWT of female RGS6 +/+ and RGS6 -/- 180 minutes after injection. F. Average MWT after an injection U50,488 over time in RGS7 +/+ and RGS7 -/- male mice (N=6). Insert is the average MWT of male RGS7 +/+ and RGS7 -/- at 180 minutes after injection. G. Average MWT necessary to elicit a pain response after an injection U50,488 over time in RGS7 +/+ and RGS7 -/- female mice (N=6-7). Insert is the average MWT of female RGS7 +/+ and RGS7 -/- 180 minutes after injection. Data shown as means ± SEM; * p≤0.05, ** p≤0.01, *** p≤0.001, **** p<0.0001.

    Techniques Used: Injection

    Role of R7 RGS protein family on KOR-induced thermal analgesia. Colors assigned to males and females are blue and green respectively. A. Schematic and timeline of hot plate with U50,488 (IP) in both sexes. B. 15 mg/kg U50,488 on KOR-induced analgesia in C57BLJ6 male and female mice (N=18-19). C. Average latency to show a pain response over time in male RGS6 +/+ and RGS6 -/- mice (N=10-9). D. Average latency to show a pain response over time in female RGS6 +/+ and RGS6 -/- mice (N=8). E. Comparison of peak analgesic response between RGS6 +/+ and RGS6 -/- in male and female mice. F. Average latency to show a pain response over time in male RGS7 +/+ and RGS7 -/- mice (N=8). G. Average latency to show a pain response over time in female RGS7 +/+ and RGS7 -/- mice (N=11-12). H. Comparison of peak analgesic response between RGS7 +/+ and RGS7 -/- in male and female mice. Data shown as means ± SEM; * p≤0.05, ** p≤0.01, **** p≤0.001.
    Figure Legend Snippet: Role of R7 RGS protein family on KOR-induced thermal analgesia. Colors assigned to males and females are blue and green respectively. A. Schematic and timeline of hot plate with U50,488 (IP) in both sexes. B. 15 mg/kg U50,488 on KOR-induced analgesia in C57BLJ6 male and female mice (N=18-19). C. Average latency to show a pain response over time in male RGS6 +/+ and RGS6 -/- mice (N=10-9). D. Average latency to show a pain response over time in female RGS6 +/+ and RGS6 -/- mice (N=8). E. Comparison of peak analgesic response between RGS6 +/+ and RGS6 -/- in male and female mice. F. Average latency to show a pain response over time in male RGS7 +/+ and RGS7 -/- mice (N=8). G. Average latency to show a pain response over time in female RGS7 +/+ and RGS7 -/- mice (N=11-12). H. Comparison of peak analgesic response between RGS7 +/+ and RGS7 -/- in male and female mice. Data shown as means ± SEM; * p≤0.05, ** p≤0.01, **** p≤0.001.

    Techniques Used: Comparison

    RGS6 and RGS7 on KOR-induced noxious cold hypersensitivity. A. Depiction of the cold plate experimental procedure. Mice were injected with either 0.9% Saline or a KOR agonist. B. Average number of jumps in male C57BLJ6 mice when treated with 5 mg/kg U50,488 compared to baseline on a temperature gradient of 30 oC , 10 oC , & 3 oC (N=9). C. Average number of jumps in female C57BLJ6 mice when treated with U50,488 compared to baseline on a temperature gradient of 30 oC , 10 oC , & 3 oC (N=7). D. Comparison of the average number of jumps at 3 oC between C57BLJ6 male and female mice. E. Average number of jumps on a 3 oC cold plate after an injection of saline or U50,488 in male RGS6 +/+ and RGS6 -/- mice (N=9). F. Average number of jumps in male RGS6 -/- mice when treated with U50,488 compared to baseline on a temperature gradient of 30 oC , 10 oC , & 3 oC . G. Average number of jumps on a 3 oC cold plate after an injection of saline or U50,488 in female RGS6 +/+ and RGS6 -/- mice (N=8). H. Average number of jumps in female RGS6 -/- mice when treated with U50,488 compared to baseline on a temperature gradient of 30 oC , 10 oC , & 3 oC . I. Comparison of the average number of jumps at 3 oC between RGS6 -/- male and female mice (N=9-8). J. Average number of jumps on a 3 oC cold plate after an injection of saline or U50,488 in male RGS7 +/+ and RGS7 -/- mice (N=7). K. Average number of jumps on a 3 oC cold plate after an injection of saline or U50,488 in female RGS7 +/+ and RGS7 -/- mice (N=5-9). Data shown as means ± SEM; * p≤0.05, ** p≤0.01, *** p≤0.001, **** p<0.0001.
    Figure Legend Snippet: RGS6 and RGS7 on KOR-induced noxious cold hypersensitivity. A. Depiction of the cold plate experimental procedure. Mice were injected with either 0.9% Saline or a KOR agonist. B. Average number of jumps in male C57BLJ6 mice when treated with 5 mg/kg U50,488 compared to baseline on a temperature gradient of 30 oC , 10 oC , & 3 oC (N=9). C. Average number of jumps in female C57BLJ6 mice when treated with U50,488 compared to baseline on a temperature gradient of 30 oC , 10 oC , & 3 oC (N=7). D. Comparison of the average number of jumps at 3 oC between C57BLJ6 male and female mice. E. Average number of jumps on a 3 oC cold plate after an injection of saline or U50,488 in male RGS6 +/+ and RGS6 -/- mice (N=9). F. Average number of jumps in male RGS6 -/- mice when treated with U50,488 compared to baseline on a temperature gradient of 30 oC , 10 oC , & 3 oC . G. Average number of jumps on a 3 oC cold plate after an injection of saline or U50,488 in female RGS6 +/+ and RGS6 -/- mice (N=8). H. Average number of jumps in female RGS6 -/- mice when treated with U50,488 compared to baseline on a temperature gradient of 30 oC , 10 oC , & 3 oC . I. Comparison of the average number of jumps at 3 oC between RGS6 -/- male and female mice (N=9-8). J. Average number of jumps on a 3 oC cold plate after an injection of saline or U50,488 in male RGS7 +/+ and RGS7 -/- mice (N=7). K. Average number of jumps on a 3 oC cold plate after an injection of saline or U50,488 in female RGS7 +/+ and RGS7 -/- mice (N=5-9). Data shown as means ± SEM; * p≤0.05, ** p≤0.01, *** p≤0.001, **** p<0.0001.

    Techniques Used: Injection, Saline, Comparison

    RGS6 and RGS7 on KOR-induced Sedation A. Schematic and timeline to test the sedative effect of 15 mg/kg U50,488 on a 3-30 RPM accelerating rotarod in both sexes. Representative colors for each sex are blue for males and green for females. B. Average time on the rotarod during baseline for both sexes of C57BLJ6 mice (N=6-7). C. Time on the rotarod (% of Baseline) over time after an injection of U50,488 in C57BLJ6 male and female mice. D. Average time on the rotarod during baseline for male RGS6 +/+ and RGS6 -/- mice (N=8). E. Time on the rotarod (% of Baseline) over time after an injection of U50,488 in male RGS6 +/+ and RGS6 -/- mice. F. Average time on the rotarod during baseline for female RGS6 +/+ and RGS6 -/- mice (N=8). G. Time on the rotarod (% of Baseline) over time after U50,488 in female RGS6 +/+ and RGS6 -/- mice. H. Average time on the rotarod during baseline for male RGS7 +/+ and RGS7 -/- mice (N=5-8). I. Time on the rotarod (% of Baseline) over time after U50,488 in male RGS7 +/+ and RGS7 -/- mice. J. Average time on the rotarod during baseline for female RGS7 +/+ and RGS7 -/- mice (N=6-8). K. Time on the rotarod (% of Baseline) over time after U50,488 in female RGS7 +/+ and RGS7 -/- mice. Data shown as means ± SEM.
    Figure Legend Snippet: RGS6 and RGS7 on KOR-induced Sedation A. Schematic and timeline to test the sedative effect of 15 mg/kg U50,488 on a 3-30 RPM accelerating rotarod in both sexes. Representative colors for each sex are blue for males and green for females. B. Average time on the rotarod during baseline for both sexes of C57BLJ6 mice (N=6-7). C. Time on the rotarod (% of Baseline) over time after an injection of U50,488 in C57BLJ6 male and female mice. D. Average time on the rotarod during baseline for male RGS6 +/+ and RGS6 -/- mice (N=8). E. Time on the rotarod (% of Baseline) over time after an injection of U50,488 in male RGS6 +/+ and RGS6 -/- mice. F. Average time on the rotarod during baseline for female RGS6 +/+ and RGS6 -/- mice (N=8). G. Time on the rotarod (% of Baseline) over time after U50,488 in female RGS6 +/+ and RGS6 -/- mice. H. Average time on the rotarod during baseline for male RGS7 +/+ and RGS7 -/- mice (N=5-8). I. Time on the rotarod (% of Baseline) over time after U50,488 in male RGS7 +/+ and RGS7 -/- mice. J. Average time on the rotarod during baseline for female RGS7 +/+ and RGS7 -/- mice (N=6-8). K. Time on the rotarod (% of Baseline) over time after U50,488 in female RGS7 +/+ and RGS7 -/- mice. Data shown as means ± SEM.

    Techniques Used: Injection

    Double knockout (dKO) of RGS6 and RGS7 on KOR-mediated behaviors. Colors assigned to males and females are blue and green respectively. A. Average number of jumps after an injection of saline or U50,488 in male RGS6 -/- /RGS7 -/- dKO mice and RGS7 -/- littermates (N= 5-9). B. Average number of jumps after an injection of saline or U50,488 in female dKO mice and RGS7 -/- littermates (N= 13-14). C. Comparison of average fold change in noxious cold induced jumps in male and female RGS6 -/- and dKO mice. Dotted line at 1 indicates no change. D. Average latency to show a pain response in a hot plate assay overtime in male dKO and RGS7 -/- littermates (N=9). E. Average latency to show a pain response in a hot plate assay over time in female dKO mice and RGS7 -/- littermates (N=8-9). F. Comparison of peak analgesic response between RGS7 -/- and dKO mice in both sexes. G. Comparison of peak analgesic response between male and female RGS6 -/- and dKO mice. H. Average time on the rotarod during baseline for male dKO mice and RGS7 -/- mice (N=8). I. Time on the rotarod (% of Baseline) over time after an injection of U50,488 in male dKO mice and RGS7 -/- mice. J. Average time on the rotarod during baseline for female dKO and RGS7 -/- mice (N=7-9). K. Time on the rotarod (% of Baseline) over time after U50,488 treatment in female dKO and RGS7 -/- mice. Data shown as means ± SEM; * p≤0.05, ** p≤0.01, *** p≤0.001, **** p<0.0001.
    Figure Legend Snippet: Double knockout (dKO) of RGS6 and RGS7 on KOR-mediated behaviors. Colors assigned to males and females are blue and green respectively. A. Average number of jumps after an injection of saline or U50,488 in male RGS6 -/- /RGS7 -/- dKO mice and RGS7 -/- littermates (N= 5-9). B. Average number of jumps after an injection of saline or U50,488 in female dKO mice and RGS7 -/- littermates (N= 13-14). C. Comparison of average fold change in noxious cold induced jumps in male and female RGS6 -/- and dKO mice. Dotted line at 1 indicates no change. D. Average latency to show a pain response in a hot plate assay overtime in male dKO and RGS7 -/- littermates (N=9). E. Average latency to show a pain response in a hot plate assay over time in female dKO mice and RGS7 -/- littermates (N=8-9). F. Comparison of peak analgesic response between RGS7 -/- and dKO mice in both sexes. G. Comparison of peak analgesic response between male and female RGS6 -/- and dKO mice. H. Average time on the rotarod during baseline for male dKO mice and RGS7 -/- mice (N=8). I. Time on the rotarod (% of Baseline) over time after an injection of U50,488 in male dKO mice and RGS7 -/- mice. J. Average time on the rotarod during baseline for female dKO and RGS7 -/- mice (N=7-9). K. Time on the rotarod (% of Baseline) over time after U50,488 treatment in female dKO and RGS7 -/- mice. Data shown as means ± SEM; * p≤0.05, ** p≤0.01, *** p≤0.001, **** p<0.0001.

    Techniques Used: Double Knockout, Injection, Saline, Comparison



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    RGS protein family on mechanical pain using an Von Frey Assay and Kappa Opioid Receptor (KOR) unbiased agonist, U50,488. A. Timeline and schematic of the Von Frey procedure. B. Key for male and female mice. C. Mechanical Withdrawal Threshold (MWT) after an injection of over time in male (N=23) and female (N=20) C57BLJ6 mice. D. Average MWT after U50,488 injection over time in RGS6 +/+ and RGS6 -/- male mice (N=11-10). Insert is the average MWT of male RGS6 +/+ and RGS6 -/- at the peak analgesic response point of 180 minutes after injection. E. Average MWT after an injection U50,488 over time in RGS6 +/+ and RGS6 -/- female mice (N= 8-10). Insert is the average MWT of female RGS6 +/+ and RGS6 -/- 180 minutes after injection. F. Average MWT after an injection U50,488 over time in RGS7 +/+ and RGS7 -/- male mice (N=6). Insert is the average MWT of male RGS7 +/+ and RGS7 -/- at 180 minutes after injection. G. Average MWT necessary to elicit a pain response after an injection U50,488 over time in RGS7 +/+ and RGS7 -/- female mice (N=6-7). Insert is the average MWT of female RGS7 +/+ and RGS7 -/- 180 minutes after injection. Data shown as means ± SEM; * p≤0.05, ** p≤0.01, *** p≤0.001, **** p<0.0001.

    Journal: bioRxiv

    Article Title: RGS6 regulates Kappa opioid receptor-mediated antinociceptive behaviors

    doi: 10.64898/2026.03.04.709600

    Figure Lengend Snippet: RGS protein family on mechanical pain using an Von Frey Assay and Kappa Opioid Receptor (KOR) unbiased agonist, U50,488. A. Timeline and schematic of the Von Frey procedure. B. Key for male and female mice. C. Mechanical Withdrawal Threshold (MWT) after an injection of over time in male (N=23) and female (N=20) C57BLJ6 mice. D. Average MWT after U50,488 injection over time in RGS6 +/+ and RGS6 -/- male mice (N=11-10). Insert is the average MWT of male RGS6 +/+ and RGS6 -/- at the peak analgesic response point of 180 minutes after injection. E. Average MWT after an injection U50,488 over time in RGS6 +/+ and RGS6 -/- female mice (N= 8-10). Insert is the average MWT of female RGS6 +/+ and RGS6 -/- 180 minutes after injection. F. Average MWT after an injection U50,488 over time in RGS7 +/+ and RGS7 -/- male mice (N=6). Insert is the average MWT of male RGS7 +/+ and RGS7 -/- at 180 minutes after injection. G. Average MWT necessary to elicit a pain response after an injection U50,488 over time in RGS7 +/+ and RGS7 -/- female mice (N=6-7). Insert is the average MWT of female RGS7 +/+ and RGS7 -/- 180 minutes after injection. Data shown as means ± SEM; * p≤0.05, ** p≤0.01, *** p≤0.001, **** p<0.0001.

    Article Snippet: KOR agonists (±)-U50,488 hydrochloride and ICI 404,488 hydrochloride were obtained from Tocris Bioscience (Bristol, UK).

    Techniques: Injection

    Role of R7 RGS protein family on KOR-induced thermal analgesia. Colors assigned to males and females are blue and green respectively. A. Schematic and timeline of hot plate with U50,488 (IP) in both sexes. B. 15 mg/kg U50,488 on KOR-induced analgesia in C57BLJ6 male and female mice (N=18-19). C. Average latency to show a pain response over time in male RGS6 +/+ and RGS6 -/- mice (N=10-9). D. Average latency to show a pain response over time in female RGS6 +/+ and RGS6 -/- mice (N=8). E. Comparison of peak analgesic response between RGS6 +/+ and RGS6 -/- in male and female mice. F. Average latency to show a pain response over time in male RGS7 +/+ and RGS7 -/- mice (N=8). G. Average latency to show a pain response over time in female RGS7 +/+ and RGS7 -/- mice (N=11-12). H. Comparison of peak analgesic response between RGS7 +/+ and RGS7 -/- in male and female mice. Data shown as means ± SEM; * p≤0.05, ** p≤0.01, **** p≤0.001.

    Journal: bioRxiv

    Article Title: RGS6 regulates Kappa opioid receptor-mediated antinociceptive behaviors

    doi: 10.64898/2026.03.04.709600

    Figure Lengend Snippet: Role of R7 RGS protein family on KOR-induced thermal analgesia. Colors assigned to males and females are blue and green respectively. A. Schematic and timeline of hot plate with U50,488 (IP) in both sexes. B. 15 mg/kg U50,488 on KOR-induced analgesia in C57BLJ6 male and female mice (N=18-19). C. Average latency to show a pain response over time in male RGS6 +/+ and RGS6 -/- mice (N=10-9). D. Average latency to show a pain response over time in female RGS6 +/+ and RGS6 -/- mice (N=8). E. Comparison of peak analgesic response between RGS6 +/+ and RGS6 -/- in male and female mice. F. Average latency to show a pain response over time in male RGS7 +/+ and RGS7 -/- mice (N=8). G. Average latency to show a pain response over time in female RGS7 +/+ and RGS7 -/- mice (N=11-12). H. Comparison of peak analgesic response between RGS7 +/+ and RGS7 -/- in male and female mice. Data shown as means ± SEM; * p≤0.05, ** p≤0.01, **** p≤0.001.

    Article Snippet: KOR agonists (±)-U50,488 hydrochloride and ICI 404,488 hydrochloride were obtained from Tocris Bioscience (Bristol, UK).

    Techniques: Comparison

    RGS6 and RGS7 on KOR-induced noxious cold hypersensitivity. A. Depiction of the cold plate experimental procedure. Mice were injected with either 0.9% Saline or a KOR agonist. B. Average number of jumps in male C57BLJ6 mice when treated with 5 mg/kg U50,488 compared to baseline on a temperature gradient of 30 oC , 10 oC , & 3 oC (N=9). C. Average number of jumps in female C57BLJ6 mice when treated with U50,488 compared to baseline on a temperature gradient of 30 oC , 10 oC , & 3 oC (N=7). D. Comparison of the average number of jumps at 3 oC between C57BLJ6 male and female mice. E. Average number of jumps on a 3 oC cold plate after an injection of saline or U50,488 in male RGS6 +/+ and RGS6 -/- mice (N=9). F. Average number of jumps in male RGS6 -/- mice when treated with U50,488 compared to baseline on a temperature gradient of 30 oC , 10 oC , & 3 oC . G. Average number of jumps on a 3 oC cold plate after an injection of saline or U50,488 in female RGS6 +/+ and RGS6 -/- mice (N=8). H. Average number of jumps in female RGS6 -/- mice when treated with U50,488 compared to baseline on a temperature gradient of 30 oC , 10 oC , & 3 oC . I. Comparison of the average number of jumps at 3 oC between RGS6 -/- male and female mice (N=9-8). J. Average number of jumps on a 3 oC cold plate after an injection of saline or U50,488 in male RGS7 +/+ and RGS7 -/- mice (N=7). K. Average number of jumps on a 3 oC cold plate after an injection of saline or U50,488 in female RGS7 +/+ and RGS7 -/- mice (N=5-9). Data shown as means ± SEM; * p≤0.05, ** p≤0.01, *** p≤0.001, **** p<0.0001.

    Journal: bioRxiv

    Article Title: RGS6 regulates Kappa opioid receptor-mediated antinociceptive behaviors

    doi: 10.64898/2026.03.04.709600

    Figure Lengend Snippet: RGS6 and RGS7 on KOR-induced noxious cold hypersensitivity. A. Depiction of the cold plate experimental procedure. Mice were injected with either 0.9% Saline or a KOR agonist. B. Average number of jumps in male C57BLJ6 mice when treated with 5 mg/kg U50,488 compared to baseline on a temperature gradient of 30 oC , 10 oC , & 3 oC (N=9). C. Average number of jumps in female C57BLJ6 mice when treated with U50,488 compared to baseline on a temperature gradient of 30 oC , 10 oC , & 3 oC (N=7). D. Comparison of the average number of jumps at 3 oC between C57BLJ6 male and female mice. E. Average number of jumps on a 3 oC cold plate after an injection of saline or U50,488 in male RGS6 +/+ and RGS6 -/- mice (N=9). F. Average number of jumps in male RGS6 -/- mice when treated with U50,488 compared to baseline on a temperature gradient of 30 oC , 10 oC , & 3 oC . G. Average number of jumps on a 3 oC cold plate after an injection of saline or U50,488 in female RGS6 +/+ and RGS6 -/- mice (N=8). H. Average number of jumps in female RGS6 -/- mice when treated with U50,488 compared to baseline on a temperature gradient of 30 oC , 10 oC , & 3 oC . I. Comparison of the average number of jumps at 3 oC between RGS6 -/- male and female mice (N=9-8). J. Average number of jumps on a 3 oC cold plate after an injection of saline or U50,488 in male RGS7 +/+ and RGS7 -/- mice (N=7). K. Average number of jumps on a 3 oC cold plate after an injection of saline or U50,488 in female RGS7 +/+ and RGS7 -/- mice (N=5-9). Data shown as means ± SEM; * p≤0.05, ** p≤0.01, *** p≤0.001, **** p<0.0001.

    Article Snippet: KOR agonists (±)-U50,488 hydrochloride and ICI 404,488 hydrochloride were obtained from Tocris Bioscience (Bristol, UK).

    Techniques: Injection, Saline, Comparison

    RGS6 and RGS7 on KOR-induced Sedation A. Schematic and timeline to test the sedative effect of 15 mg/kg U50,488 on a 3-30 RPM accelerating rotarod in both sexes. Representative colors for each sex are blue for males and green for females. B. Average time on the rotarod during baseline for both sexes of C57BLJ6 mice (N=6-7). C. Time on the rotarod (% of Baseline) over time after an injection of U50,488 in C57BLJ6 male and female mice. D. Average time on the rotarod during baseline for male RGS6 +/+ and RGS6 -/- mice (N=8). E. Time on the rotarod (% of Baseline) over time after an injection of U50,488 in male RGS6 +/+ and RGS6 -/- mice. F. Average time on the rotarod during baseline for female RGS6 +/+ and RGS6 -/- mice (N=8). G. Time on the rotarod (% of Baseline) over time after U50,488 in female RGS6 +/+ and RGS6 -/- mice. H. Average time on the rotarod during baseline for male RGS7 +/+ and RGS7 -/- mice (N=5-8). I. Time on the rotarod (% of Baseline) over time after U50,488 in male RGS7 +/+ and RGS7 -/- mice. J. Average time on the rotarod during baseline for female RGS7 +/+ and RGS7 -/- mice (N=6-8). K. Time on the rotarod (% of Baseline) over time after U50,488 in female RGS7 +/+ and RGS7 -/- mice. Data shown as means ± SEM.

    Journal: bioRxiv

    Article Title: RGS6 regulates Kappa opioid receptor-mediated antinociceptive behaviors

    doi: 10.64898/2026.03.04.709600

    Figure Lengend Snippet: RGS6 and RGS7 on KOR-induced Sedation A. Schematic and timeline to test the sedative effect of 15 mg/kg U50,488 on a 3-30 RPM accelerating rotarod in both sexes. Representative colors for each sex are blue for males and green for females. B. Average time on the rotarod during baseline for both sexes of C57BLJ6 mice (N=6-7). C. Time on the rotarod (% of Baseline) over time after an injection of U50,488 in C57BLJ6 male and female mice. D. Average time on the rotarod during baseline for male RGS6 +/+ and RGS6 -/- mice (N=8). E. Time on the rotarod (% of Baseline) over time after an injection of U50,488 in male RGS6 +/+ and RGS6 -/- mice. F. Average time on the rotarod during baseline for female RGS6 +/+ and RGS6 -/- mice (N=8). G. Time on the rotarod (% of Baseline) over time after U50,488 in female RGS6 +/+ and RGS6 -/- mice. H. Average time on the rotarod during baseline for male RGS7 +/+ and RGS7 -/- mice (N=5-8). I. Time on the rotarod (% of Baseline) over time after U50,488 in male RGS7 +/+ and RGS7 -/- mice. J. Average time on the rotarod during baseline for female RGS7 +/+ and RGS7 -/- mice (N=6-8). K. Time on the rotarod (% of Baseline) over time after U50,488 in female RGS7 +/+ and RGS7 -/- mice. Data shown as means ± SEM.

    Article Snippet: KOR agonists (±)-U50,488 hydrochloride and ICI 404,488 hydrochloride were obtained from Tocris Bioscience (Bristol, UK).

    Techniques: Injection

    Double knockout (dKO) of RGS6 and RGS7 on KOR-mediated behaviors. Colors assigned to males and females are blue and green respectively. A. Average number of jumps after an injection of saline or U50,488 in male RGS6 -/- /RGS7 -/- dKO mice and RGS7 -/- littermates (N= 5-9). B. Average number of jumps after an injection of saline or U50,488 in female dKO mice and RGS7 -/- littermates (N= 13-14). C. Comparison of average fold change in noxious cold induced jumps in male and female RGS6 -/- and dKO mice. Dotted line at 1 indicates no change. D. Average latency to show a pain response in a hot plate assay overtime in male dKO and RGS7 -/- littermates (N=9). E. Average latency to show a pain response in a hot plate assay over time in female dKO mice and RGS7 -/- littermates (N=8-9). F. Comparison of peak analgesic response between RGS7 -/- and dKO mice in both sexes. G. Comparison of peak analgesic response between male and female RGS6 -/- and dKO mice. H. Average time on the rotarod during baseline for male dKO mice and RGS7 -/- mice (N=8). I. Time on the rotarod (% of Baseline) over time after an injection of U50,488 in male dKO mice and RGS7 -/- mice. J. Average time on the rotarod during baseline for female dKO and RGS7 -/- mice (N=7-9). K. Time on the rotarod (% of Baseline) over time after U50,488 treatment in female dKO and RGS7 -/- mice. Data shown as means ± SEM; * p≤0.05, ** p≤0.01, *** p≤0.001, **** p<0.0001.

    Journal: bioRxiv

    Article Title: RGS6 regulates Kappa opioid receptor-mediated antinociceptive behaviors

    doi: 10.64898/2026.03.04.709600

    Figure Lengend Snippet: Double knockout (dKO) of RGS6 and RGS7 on KOR-mediated behaviors. Colors assigned to males and females are blue and green respectively. A. Average number of jumps after an injection of saline or U50,488 in male RGS6 -/- /RGS7 -/- dKO mice and RGS7 -/- littermates (N= 5-9). B. Average number of jumps after an injection of saline or U50,488 in female dKO mice and RGS7 -/- littermates (N= 13-14). C. Comparison of average fold change in noxious cold induced jumps in male and female RGS6 -/- and dKO mice. Dotted line at 1 indicates no change. D. Average latency to show a pain response in a hot plate assay overtime in male dKO and RGS7 -/- littermates (N=9). E. Average latency to show a pain response in a hot plate assay over time in female dKO mice and RGS7 -/- littermates (N=8-9). F. Comparison of peak analgesic response between RGS7 -/- and dKO mice in both sexes. G. Comparison of peak analgesic response between male and female RGS6 -/- and dKO mice. H. Average time on the rotarod during baseline for male dKO mice and RGS7 -/- mice (N=8). I. Time on the rotarod (% of Baseline) over time after an injection of U50,488 in male dKO mice and RGS7 -/- mice. J. Average time on the rotarod during baseline for female dKO and RGS7 -/- mice (N=7-9). K. Time on the rotarod (% of Baseline) over time after U50,488 treatment in female dKO and RGS7 -/- mice. Data shown as means ± SEM; * p≤0.05, ** p≤0.01, *** p≤0.001, **** p<0.0001.

    Article Snippet: KOR agonists (±)-U50,488 hydrochloride and ICI 404,488 hydrochloride were obtained from Tocris Bioscience (Bristol, UK).

    Techniques: Double Knockout, Injection, Saline, Comparison